Correlative Array Tomography - from 2D towards 3D
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چکیده
منابع مشابه
Correlative Array Tomography – from 2D towards 3D
Correlative microscopy combines information from light and electron microscopy (LM and EM) into one comprehensive dataset. The “Shuttle & Find” interface from Zeiss was the first easy to use solution for imaging one and the same sample region in different microscopes, i.e. from widefield or confocal LMs to environmental or field emission SEMs (FE-SEM). However, Shuttle & Find is limited to 2D a...
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The next step will be to address correlative 3D applications. To achieve this, it is necessary to exactly define volumes of interest (VOI) in the data of the first microscope. Further, the precise relocation of the identical VOI in the second microscope is essential as well as the registration of the 3D object in all spatial directions. However, the correlation of 3-dimensional data from differ...
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Assessment of three-dimensional morphological structure and synaptic connectivity is essential for a comprehensive understanding of neural processes controlling behavior. Different microscopy approaches have been proposed based on light microcopy (LM), electron microscopy (EM), or a combination of both. Correlative array tomography (CAT) is a technique in which arrays of ultrathin serial sectio...
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Increasingly researchers are looking to bring together perspectives across multiple scales, or to combine insights from different techniques, for the same region of interest. To this end, correlative microscopy has already yielded substantial new insights in two dimensions (2D). Here we develop correlative tomography where the correlative task is somewhat more challenging because the volume of ...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2015
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927615008612